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首页> 外文OA文献 >A Novel Septin-Associated Protein, Syp1p, Is Required for Normal Cell Cycle-Dependent Septin Cytoskeleton Dynamics in Yeast
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A Novel Septin-Associated Protein, Syp1p, Is Required for Normal Cell Cycle-Dependent Septin Cytoskeleton Dynamics in Yeast

机译：酵母中正常细胞周期依赖性Septin细胞骨架动力学需要一种新型的Septin相关蛋白Syp1p。

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摘要

Septins are a family of GTP-binding proteins whose heterooligomeric complex is the basic structural element of the septin filaments found in many eukaryotic organisms. In budding yeast, septins are mainly confined at the mother–daughter junction and are required for cell morphogenesis and division. Septins undergo assembly and disassembly in accordance with the progression of the cell cycle. In this report, we identified the yeast protein Syp1p as a new regulator of septin dynamics. Syp1p colocalizes with septins throughout most of the cell cycle. Syp1p interacts with the septin subunit Cdc10p and can be precipitated by Cdc10p and Cdc12p. In the syp1Δ mutant, both formation of a complete septin ring at the incipient bud site and disassembly of the septin ring in later stages of cell division are significantly delayed. In addition, overexpression of Syp1p causes marked acceleration of septin disassembly. The fluorescence recovery after photobleaching (FRAP) assay further showed that Syp1p promotes septin turnover in different cell cycle stages. These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast.
机译：Septins是GTP结合蛋白的一个家族，其杂合寡聚复合物是在许多真核生物中发现的septin丝的基本结构元件。在发芽酵母中，Septins主要限制在母子交界处，是细胞形态发生和分裂所必需的。隔膜根据细胞周期的进程进行组装和拆卸。在这份报告中，我们确定了酵母蛋白Syp1p是Septin动力学的新调节剂。在整个细胞周期的大部分时间内，Syp1p与Septins共定位。 Syp1p与Septin亚基Cdc10p相互作用，并且可以被Cdc10p和Cdc12p沉淀。在syp1Δ突变体中，在初始芽位点形成完整的septin环和在细胞分裂后期均会明显延迟septin环的拆卸。此外，Syp1p的过表达会导致隔膜分离的明显加速。光漂白后的荧光恢复（FRAP）分析进一步表明Syp1p促进不同细胞周期阶段的septin更新。这些结果表明，Syp1p参与了酵母中Septin细胞骨架的细胞周期依赖性动力学的调控。

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